PDMS plate casting mold assembly and PDMS plate casting On this note, we would advise against using Fused Deposition Modeling (FDM) which cannot withstand required curing temperature.Ĭhemicals, peptides, and recombinant proteinsġ-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) If the end user decides to use 3D printing, we would strongly advise to consider some of the potential drawbacks and limitations of this technology, and seek expert advice regarding choice of printing technology, material, etc. Nevertheless, 3D printing now emerges as a very efficient alternative to other methods which, we thought, was worth mentioning. The authors only used plexiglass molds and never experienced 3D printing of these molds. The longer timing takes into account ordering, production, and shipping when ordering from a remote supplier. Note: A timing of a few days was indicated but this step can be achieved faster if direct access to a workshop or a 3D printer is available in which case a few hours should be enough. Troubleshooting 4 and 5Īssemble the casting mold using M2 screwsĬRITICAL: Upon assembly of the casting mold and in order to prevent PDMS leakage, tape the exterior of the casting mold with two rounds of lab tape.Īdditionally, the casting molds, at least the plexiglass molds, can be cleaned and re-used indefinitely. The extent of stretch can also be adjusted by modifying the length of the main arm axle, however, in such case, de novo full characterization of the stretcher motion is required. The frequency of the stretch can be adjusted by step increments between 0.2 to 1Hz ( Table 1) by changing the combination of wheels of the gear box as indicated in ( Boulter et al., 2020). Note: Precise characterization of the mechanical stretcher and presentation of all its features are reported in the original publication. This mostly includes adding the motors at step 80 and gluing the 8-stud axle in the final step (86). We estimate the assembly time to be one to two hours per stretcher.ĬRITICAL: During the assembly of the LEGO® stretcher, the critical steps are commented in the assembly booklet. Assemble LEGO® mechanical stretcher according to instructions from the assembly manual (see supplemental file 2 available at ) with help from the Studio 2.0 model files when necessary (see supplemental file 3–5 available at ).

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